Chemical and Biological Diversity of the Leaf and Rhizome Volatiles of Acorus calamus L. from Turkey

SÜZGEÇ SELÇUK S., Ozek G., Mericli A. H., Baser K. H. C., Haliloglu Y., ÖZEK T.

JOURNAL OF ESSENTIAL OIL BEARING PLANTS, cilt.20, sa.3, ss.646-661, 2017 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 20 Sayı: 3
  • Basım Tarihi: 2017
  • Doi Numarası: 10.1080/0972060x.2017.1331142
  • Dergi Adı: JOURNAL OF ESSENTIAL OIL BEARING PLANTS
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.646-661
  • Anahtar Kelimeler: Acorus calamus, volatiles, beta-asarone, acetylcholinesterase, antioxidant, ACETYLCHOLINESTERASE INHIBITORY-ACTIVITY, ESSENTIAL OIL COMPOSITION, BETA-ASARONE, ANTIOXIDANT CAPACITY, SWEET FLAG, IN-VITRO, PLANTS, ASSAY, IDENTIFICATION, CHROMATOGRAPHY
  • Anadolu Üniversitesi Adresli: Evet

Özet

Two different isolation techniques, conventional hydrodistillation (HD) and micro-steam distillation-solid-phase microextraction (MSD-SPME), have been used to analyze the volatile constituents from the leaves and rhizomes of Acorus calamus L. by gas chromatography (GC-FID) and gas chromatography coupled to mass spectrometry (GC-MS). In all the samples studied, phenylpropanoids (16.9-35.3 %) predominated by beta-asarone (15.3-16.3 % in rhizomes, 23.4-31.7 % in leaves) were the main constituent. In addition to phenylpropanoids, the acorane type sesquiterpene acorenone B (7.4-16.4 %) and elemene-type sesquiterpenes, epi-isoshyobunone (3.3-7.3 %) and shyobunone (1.5-4.6 %) were detected among the major volatile constituents in the rhizomes. The monoterpenes were represented by camphor (7.5-13.9 %) and camphene (6.1-7.7 %). In the leaf, myrcene (0.3-7.1 %), limonene (1.0-5.6 %), (Z)-beta-ocimene (2.9-6.1 %) were among the major constituents. Qualitative difference of the volatiles composition in the rhizomes and leaves are discussed as well as of the volatiles obtained by HD and MSD-SPME techniques are discussed. Biological activity tests resulted in moderate anti-acetylcholinesterase effect and significant cupric reducing antioxidant potential of the leaf oil, while the rhizome oil demonstrated relatively lower effects.