The effects of stocking density on CYP 450 1A gene expression and carbonic anhydrase enzyme activity in rainbow trout (Oncorhynchus mykiss)


BEYDEMİR Ş., AKSAKAL E., Alim Z., ERDOĞAN O., CEYHUN S. B.

Fresenius Environmental Bulletin, vol.20, no.6, pp.1452-1457, 2011 (SCI-Expanded) identifier

  • Publication Type: Article / Article
  • Volume: 20 Issue: 6
  • Publication Date: 2011
  • Journal Name: Fresenius Environmental Bulletin
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.1452-1457
  • Keywords: Stocking density; gene expression; multiplex real time pcr; cytochrome p450; carbonic anhydrase; rainbow trout
  • Anadolu University Affiliated: Yes

Abstract

It is well-known that environmental factors cause some changes in metabolisms of all living organisms. Such metabolic changes may take place in genetic structure, antioxidant mechanism, certain enzyme activities etc. The aim of this study was to assess the effects of increasing stock density on the activity of carbonic anhydrase (CA) enzyme in liver, muscle, gill and kidney tissues, and CYP 450 1A gene expression in muscle tissue of rainbow trout. For this purpose, we prepared four groups of trout: the control group (15 kg/m 3 ) and three experimental groups of 20, 25, 30 kg/m3. CA activities were determined from tissue homogenates. Interestingly, an increase was observed in gill CA activity. The studies, particularly those on gill CA activity, showed that there could be an important correlation between CA activity and stocking density, and oxygen consumption of rainbow trout. The results about CYP 450 1A gene expression demonstrated that expression of the gene significantly increases with stocking density. © by PSP.