DNA authentication of plantago herb based on nucleotide sequences of 18S-28S rRNA internal transcribed spacer region

Sahin F. P., Yamashita H., Guo Y., Terasaka K., Kondo T., Yamamoto Y., ...More

Biological and Pharmaceutical Bulletin, vol.30, no.7, pp.1265-1270, 2007 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 30 Issue: 7
  • Publication Date: 2007
  • Doi Number: 10.1248/bpb.30.1265
  • Journal Name: Biological and Pharmaceutical Bulletin
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.1265-1270
  • Keywords: DNA authentication, Internal transcribed spacer, Internal transcribed spacer (ITS), Plantago herb, Ribosomal DNA
  • Anadolu University Affiliated: No

Abstract

Internal transcribed spacer (ITS) regions of nuclear ribosomal RNA gene were amplified from 23 plant- and herbarium specimens belonging to eight Plantago species (P. asiatica, P. depressa, P. major, P. erosa, P. hostifolia, P. camtschatica, P. virginica and P. lanceolata). Sequence comparison indicated that these Plantago species could be identified based on the sequence type of the ITS locus. Sequence analysis of the ITS regions amplified from the crude drug Plantago Herb obtained in the markets indicated that all the drugs from Japan were derived from P. asiatica whereas the samples obtained in China were originated from various Plantago species including P. asiatica, P. depressa, P. major and P. erosa. © 2007 Pharmaceutical Society of Japan.