Design, Synthesis, In Vitro and In Silico Studies of New Thiazolylhydrazine-Piperazine Derivatives as Selective MAO-A Inhibitors

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SAĞLIK B. N., Cebeci O., ACAR ÇEVİK U., OSMANİYE D., LEVENT S., Kaya cavusoglu B., ...Daha Fazla

MOLECULES, cilt.25, sa.18, 2020 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 25 Sayı: 18
  • Basım Tarihi: 2020
  • Doi Numarası: 10.3390/molecules25184342
  • Dergi Adı: MOLECULES
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Aerospace Database, Agricultural & Environmental Science Database, Biotechnology Research Abstracts, CAB Abstracts, Chemical Abstracts Core, Communication Abstracts, EMBASE, MEDLINE, Metadex, Veterinary Science Database, Directory of Open Access Journals, Civil Engineering Abstracts
  • Anahtar Kelimeler: ADME properties, in vitro enzyme inhibition, molecular docking, monoamine oxidases, thiazolylhydrazine, piperazine, MONOAMINE-OXIDASE, SOLUBILITY, POTENT
  • Anadolu Üniversitesi Adresli: Evet

Özet

Monoamine oxidase (MAO) isoenzymes are very important drug targets among neurological disorders. Herein, novel series of thiazolylhydrazine-piperazine derivatives were designed, synthesized and evaluated for their MAO-A and -B inhibitory activity. The structures of the synthesized compounds were assigned using different spectroscopic techniques such as(1)H-NMR,C-13-NMR and HRMS. Moreover, the prediction of ADME (Absorption, Distribution, Metabolism, Elimination) parameters for all of the compounds were performed using in silico method. According to the enzyme inhibition results, the synthesized compounds showed the selectivity against MAO-A enzyme inhibition. Compounds3c,3dand3edisplayed significant MAO-A inhibition potencies. Among them, compound3ewas found to be the most effective derivative with an IC(50)value of 0.057 +/- 0.002 mu M. Moreover, it was seen that this compound has a more potent inhibition profile than the reference inhibitors moclobemide (IC50= 6.061 +/- 0.262 mu M) and clorgiline (IC50= 0.062 +/- 0.002 mu M). In addition, the enzyme kinetics were performed for compound3eand it was determined that this compound had a competitive and reversible inhibition type. Molecular modeling studies aided in the understanding of the interaction modes between this compound and MAO-A. It was found that compound 3e had significant and important binding property.