Native to the Mediterranean region, Laurus nobilis L. (Lauraceae) is cultivated extensively throughout Southern Europe, Western Asia, Northern Africa, and America [1,2]. Traditional uses for the leaves and fruits include the treatment of rheumatism, heart conditions, viral infections, diarrhea, as well as their carminative, diaphoretic, and antiseptic characteristics [2]. In this study, gels containing L. nobilis essential oil were prepared and characterized primarily in terms of pharmaceutical technology (rheology, pH etc.) [3]. Then, the selected optimum formulation was evaluated in terms of in vitro antioxidant, in vivo cosmetic irritant potency and embryotoxicity. The GC-FID/MS method was used to screen the composition of the extracted essential oil from the leaves. By employing in vitro DPPH scavenging, metal chelating potential and in vivo HET-CAM (hen's egg-chorioallantoic membrane test) assay respectively, in vitro antioxidant capacities and in vivo cosmetic irritation potential of the gel on the CAM (chorioallantoic membrane) were examined. The two main constituents of the essential oil were identified as 1,8-cineole (47.1%) and α-terpinyl acetate (17.9%). The gel’s IC50 value for DPPH scavenging and for metal chelating were calculated as 0.228 and 0.66 mg.mL-1, respectively. When compared to SDS, the gels did not exhibit any irritation or embryotoxicity on the CAM. This study describes a promising topical gel with L. nobilis essential oil that has strong antioxidant properties in vitro and no cosmetic irritation with no embryotoxicity in vivo.