Cytotoxic and genotoxic effects of [Ru(phi)(3)](2+) evaluated by Ames/Salmonella and MTT methods

BENKLİ K., TUNALI Y., CANTÜRK Z., Artagan O., ALANYALI F.

EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY, cilt.44, sa.6, ss.2601-2605, 2009 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 44 Sayı: 6
  • Basım Tarihi: 2009
  • Doi Numarası: 10.1016/j.ejmech.2008.09.017
  • Dergi Adı: EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Index Chemicus (IC)
  • Sayfa Sayıları: ss.2601-2605
  • Anahtar Kelimeler: 9,10-Phenanthrenequinone diimine, Ru(II) complex, Mutagenicity, Cytotoxic effect, Genotoxicity, DNA-BINDING, METAL-COMPLEXES, RUTHENIUM(II), SPECIFICITY
  • Anadolu Üniversitesi Adresli: Evet

Özet

In this work, we synthesized and evaluated the cytotoxic effect of [Ru(phi)(3)](2+), on rat C6 glioma cell line. Cell viability was determined by assay with 3-(4-5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT). The mutagenicity of [Ru(phi)(3)](2+) was studied in vitro by using two strains of Salmonella typhimurium with frameshift mutation (TA98) and base-pair substitution mutation (TA100) were used in plate incorporation assay in the absence of metabolic activation. According to the results, the Ru compound is not toxic but mutagenic, and it shows cytotoxic effect towards C6 rat glioma cells in 100 mu M. (C) 2008 Elsevier Masson SAS. All rights reserved.