A SIMPLE AND SPECIFIC HPLC METHOD FOR THE DETERMINATION OF ATOMOXETINE IN PHARMACEUTICALS AND HUMAN PLASMA


Dogrukol-Ak D., YENİCELİ UĞUR D.

JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES, vol.33, no.19, pp.1745-1759, 2010 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 33 Issue: 19
  • Publication Date: 2010
  • Doi Number: 10.1080/10826076.2010.526816
  • Journal Name: JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.1745-1759
  • Keywords: atomoxetine, high performance liquid chromatography, human plasma, method validation, pharmaceutical analysis, protein precipitation, LIQUID-CHROMATOGRAPHIC METHOD, QUANTIFICATION, HYDROCHLORIDE, VALIDATION, PHASE, DRUG
  • Anadolu University Affiliated: Yes

Abstract

A liquid chromatographic method has been described for the determination of atomoxetine in pharmaceuticals and human plasma. Plasma samples were analyzed after a simple, one step protein precipitation with methanol, and chromatographic separation of atomoxetine and carbamazepine (internal standard) was carried out using the optimum mobile phase of a methanol/acetonitrile/phosphate buffer (10mM, pH 3.0) (35:15:50, v/v/v). Limit of quantification values were 45.2 and 49.5ng/mL for atomoxetine in the mobile phase and human plasma, respectively. Fully validated method is reproducible and selective for the determination of atomoxetine in pharmaceuticals and human plasma.