Experiments were designed to evaluate the usefulness of Rhizobium sp. obtained from clover root nodules in the soil where fungicides, antibiotics and NaCI concentrations presence. The effect of fungicides, antibiotics and NaCI concentrations were variable depending on Rhizobium sp. strains tested. Rhizobium CY8, CY11 and CY12 strains had the best performance of growth in all tested conditions. Agarose gel electrophoresis using the Miniprep method for plasmid isolation revealed that tested strains harboured plasmids of molecular weight 125 bp -2027 bp. © by PSP Volume 18-No 5a. 2009.