Lab-on-a-chip devices have now-a-days become an important aspect of analytical/bioanalytical chemistry having wide range of applications including clinical diagnosis, drug screening, cell biology, environmental monitoring, food safety analysis etc. Conventional lab-on-a-chip devices generally employ chemicals that are not environmentally friendly and were commonly fabricated on hard plastic platform which are non-degradable and hence ignore the importance of green analytical chemistry. In today's scenario, it is highly imperative to protect our environment by using less toxic and environmentally friendly chemicals/solvents and biocompatible platforms. Accordingly, the present article comprehensively reviews on the various green aspects of lab-on-a-chip devices for analytical processes which aim at fabricating environmentally friendly and cost-effective downsized devices so that the risk factor at the user's end upon longer exposure as well as to the environment can be reduced. The decisive factors for the accomplishment of green aspects of lab-on-a-chip devices including sample preparation using lab-on-a-chip systems to minimize the amount of sample/solvents to few microliters only, substitution of harmful solvents with green alternatives, minimal waste generation or proper treatment of waste and biodegradable and biocompatible platforms for fabricating lab-on-a-chip devices have been discussed in details. Additionally, the challenges that may hinder their commercialization are also critically discussed.