Enzyme inhibitory and antioxidant activity of Melia azedarach L. naturalized in Anatolia and its phenolic acid and fatty acid composition

ERDOĞAN ORHAN İ., Guner E., Ozturk N., ŞENOL DENİZ F. S., ASLAN ERDEM S., Kartal M., ...More

INDUSTRIAL CROPS AND PRODUCTS, vol.37, no.1, pp.213-218, 2012 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 37 Issue: 1
  • Publication Date: 2012
  • Doi Number: 10.1016/j.indcrop.2011.11.028
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.213-218
  • Keywords: Melia azedarach, Enzyme inhibition, Antioxidant activity, Phenolic acid, Fatty acid, MEDICINAL-PLANTS, EXTRACTS, ACETYLCHOLINESTERASE, CHOLINESTERASE, PRODUCTS
  • Anadolu University Affiliated: Yes


In the present study, acetylcholinesterase (AChE), butyrylcholinesterase (BChE), and tyrosinase inhibitory and antioxidant activities of the fruit and leaf extracts of Melia azedarach L (Meliaceae) of Turkish origin were evaluated. Enzyme inhibitory activity of the extracts was tested in vitro using ELISA microplate reader. Antioxidant activity of the extracts was tested using 2,2-dipheny1-1-picrylhydrazyl (DPPH) radical scavenging, ferrous ion-chelation, and ferric-reducing antioxidant power (FRAP) assays. Phenolic composition of the extracts was elucidated by high performance liquid chromatography (HPLC) and fatty acid compositions of the fatty oils of the fruits and leaves were elucidated by gas chromatography-mass spectrometry (GC-MS). The ethyl acetate extract from the leaves showed the highest inhibition against AChE (33.63 +/- 1.40%) and BChE (92.89 +/- 3.05%). The methanol extract from the leaves exerted the best antioxidant activity in DPPH radical scavenging and FRAP assays, while the ethyl acetate extracts of the fruits and leaves had the most notable effect in metal-chelation assay. (C) 2011 Elsevier B.V. All rights reserved.