In Vitro and In Vivo Evaluation of Pogostemon cablin Essential Oil Loaded Nanoemulgel for Wound Healing

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Öztürk A. A., Güven U. M., Demirci Kayıran S.

JOINT EUFEPS - SITELF MEETING “Advances in Pharmaceutical Sciences”, Naples, Italy, 27 - 29 May 2026, no.76, pp.76, (Summary Text)

  • Publication Type: Conference Paper / Summary Text
  • City: Naples
  • Country: Italy
  • Page Numbers: pp.76
  • Anadolu University Affiliated: Yes

Abstract

Impaired wound healing is frequently associated with excessive inflammation, inadequate angiogenesis, and insufficient extracellular matrix remodeling [1]. Plant derived essential oils have attracted increasing interest as natural therapeutic agents due to their antiinflammatory, antimicrobial, and regenerative properties [2]. A nanoemulgel formulation of Pogostemon cablin essential oil was prepared and its wound healing activity was showcased in this study. Characterization of the prepared nanoemulsion and chitosan based nanoemulgel systems were performed for their physicochemical properties such as droplet size distribution, zeta potential, pH, viscosity and rheological behavior [3]. Fibroblast cell lines were used to evaluate the cytocompatibility of the formulations. In depth, in vivo wound healing activity was studied using experimental burn wound model by analyzing percentage of wound contraction, histopathological changes and key molecular signals involved in tissue repair. ELISA analyses were conducted to determine the levels of inflammatory cytokines, angiogenic growth factors and hydroxyproline as an indicator of collagen deposition. Treatment with the P. cablin essential oil nanoemulgel significantly accelerated wound closure compared with the control group. Histological findings demonstrated enhanced reepithelialization, improved collagen organization, and increased angiogenic activity in the treated tissues. These effects were accompanied by decreased proinflammatory cytokine levels and increased VEGF, TGF-β, and hydroxyproline levels. Overall, the results suggest that the P. cablin essential oil loaded nanoemulgel may represent a promising plant based therapeutic approach for promoting wound healing through modulation of inflammation, angiogenesis, and extracellular matrix remodeling.