Multiclonal plastic antibodies for selective aflatoxin extraction from food samples


Bayram E., Yilmaz E., UZUN L., Say R., DENİZLİ A.

FOOD CHEMISTRY, cilt.221, ss.829-837, 2017 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 221
  • Basım Tarihi: 2017
  • Doi Numarası: 10.1016/j.foodchem.2016.11.090
  • Dergi Adı: FOOD CHEMISTRY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.829-837
  • Anahtar Kelimeler: Molecular imprinting, Multiclonal plastic antibodies, Simultaneous imprinting, Cryogels, Aflatoxin extraction, Food samples, MOLECULARLY-IMPRINTED POLYMER, IMMUNOGLOBULIN-G RECOGNITION, SOLID-PHASE EXTRACTION, LIQUID-CHROMATOGRAPHY, CRYOGELS, ADSORPTION, CONTAMINANTS, OCHRATOXIN, MYCOTOXIN, TOXICITY
  • Anadolu Üniversitesi Adresli: Evet

Özet

Herein, we focused on developing a new generation of monolithic columns for extracting aflatoxin from real food samples by combining the superior features of molecularly imprinted polymers and cryogels. To accomplish this, we designed multiclonal plastic antibodies through simultaneous imprinting of aflatoxin subtypes B1, B2, G1, and G2. We applied Fourier transform infrared (FTIR) spectroscopy, scanning electron microscopy (SEM), and spectrofluorimetry to characterize the materials, and conducted selectivity studies using ochratoxin A and aflatoxin M1 (a metabolite of aflatoxin B1), as well as other aflatoxins, under competitive conditions. We determined optimal aflatoxin extraction conditions in terms of concentration, flow rate, temperature, and embedded particle amount as up to 25 ng/mL for each species, 0.43 mL/min, 7.0, 30 degrees C, and 200 mg, respectively. These multiclonal plastic antibodies showed imprinting efficiencies against ochratoxin A and aflatoxin M1 of 1.84 and 26.39, respectively, even under competitive conditions. Finally, we tested reusability, repeatability, reproducibility, and robustness of columns throughout inter- and intra- column variation studies. (C) 2016 Elsevier Ltd. All rights reserved.