Effect of Horse-chestnut seed extract on matrix metalloproteinase-1 and-9 during diabetic wound healing

AKSOY H., ÇEVİK Ö., ŞEN A., GÖGER F., ŞEKERLER T., ŞENER A.

JOURNAL OF FOOD BIOCHEMISTRY, cilt.43, sa.3, 2019 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 43 Sayı: 3
  • Basım Tarihi: 2019
  • Doi Numarası: 10.1111/jfbc.12758
  • Dergi Adı: JOURNAL OF FOOD BIOCHEMISTRY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Anahtar Kelimeler: diabetic wound, Horse chestnut seed extract, LC-MS/MS, matrix metalloproteinase-1, matrix metalloproteinase-9, wound healing, AESCULUS-HIPPOCASTANUM L., PHENOLIC-COMPOUNDS, OXIDATIVE STRESS, LEAF TISSUES, ANTIOXIDANT, EXPRESSION, QUERCETIN, EFFICACY, INHIBITOR, AESCIN
  • Anadolu Üniversitesi Adresli: Evet

Özet

The effects of aqueous-ethanol extract of Horse chestnut (HCE) on MMP-1 and MMP-9 expressions during cutaneous wound healing in diabetic rats were investigated in this study. The expressions of MMP-1 and MMP-9, wound closure, myeloperoxidase (MPO) activity, hydroxyproline, and malondialdehyde (MDA) levels in wound tissue were measured. Quercetin glucuronide in HCE was identified as main compound using a LC-MS/MS. The hydroxyproline level was significantly increased in the treated group versus control after the 3rd and 7th days (p < 0.05). The MDA level and MPO activity were significantly lower in the treatment group (p < 0.05). MMP-1 gene expression level in treated rats was increased in the 7th day while it was reduced in 14th day. MMP-9 gene expression level in treated rats was decreased in 7th, and 14th days compared to control (p < 0.05). These results show that HCE accelerated the cutaneous wound-healing process in diabetic rats via MMP-1 and MMP-9 regulation.