As many of the metalloid-based pollutants, the boron (B) toxicity issues have aroused more and more global attentions, especially concerning drinking water sources which flow through boron-rich areas. Therefore, feasible and innovative approaches are required in order to assess B toxicity in aquatic ecosystems. In this study, the toxic effects of B on Lemna minor L and Lemna gibba L were investigated using various endpoints including number of fronds, growth rates, dry biomass and antioxidants enzymatic activities. Lemna species were exposed to B concentrations of 2 (control), 4, 8, 16, 32, 64 and 128 mg L-1 for a test period of 7 days. The results demonstrated that plant growth was significantly reduced when the B concentration reached 16 mg L-1. Furthermore, our results also concluded that among the anti oxidative enzymes, SOD, APX and GPX can serve as important biomarkers for B-rich environment. The present results suggested that L. minor and L gibba are very useful model plants for phytoremediation of low-B contaminated wastewater and they are also suitable options for B biomonitoring due to high phototoxic sensitivity against B. In this respect, the scientific insight of the present study is to fill the gaps in the research about the use of L minor and L gibba in ecotoxicological research associated with B toxicity. (C) 2016 Elsevier Ltd. All rights reserved.