Teos, one of the twelve Ion cities, is located in the of Sigacik neighborhood Seferihisar district, 60 km southwest of Izmir. The city, on the southern coast of the peninsula Erythrea and Izmir, is the bottleneck of the gulf sea of Samos. West of the city Erythrai (Ildiri), north Klazomenai (Urla), the south island of Samos is located in the southeast of the city of Colophon. The main city is on the black neck which is surrounded by filled gulf formerly named as Teos Gulf, now west of the gulf. In ancient times the city was surrounded by walls for defense purposes. The city walls provide important information about both political and economical structure of the city. Teos walls of Hellenistic Period around 65 hectares of area is surrounded by the city for about 4 km. In this study stone samples which were used in Hellenistic Period have been characterised. Stone samples have been analysed by the following methods: field observations (visual examinations), scanning electron microscope equipped with energy dispersive X-ray spectroscopy, X-ray diffraction and chemical analysis X-ray fluorescence methods. These methods provided us determination of stone properties.