Isolation of Quality Total RNA from the Aromatic Plant Origanum onites


POYRAZ İ., SÖZEN E., ARSLANYOLU M.

ZEITSCHRIFT FUR NATURFORSCHUNG SECTION C-A JOURNAL OF BIOSCIENCES, vol.65, no.3-4, pp.266-270, 2010 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 65 Issue: 3-4
  • Publication Date: 2010
  • Doi Number: 10.1515/znc-2010-3-415
  • Journal Name: ZEITSCHRIFT FUR NATURFORSCHUNG SECTION C-A JOURNAL OF BIOSCIENCES
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.266-270
  • Keywords: RNA Isolation, Origanum onites, Secondary Metabolites, ESSENTIAL OILS, EFFICIENT PROTOCOL, TISSUES RICH, EXTRACTION
  • Anadolu University Affiliated: Yes

Abstract

We successfully used the guanidine isothiocyanate method for isolation of total RNA from leaf, stem, and root tissues of the aromatic plant Origanum onites. The RNA was extracted with TRI Reagent (R) at room temperature and was recovered by isopropanol precipitation. The isolated RNA was capable of reverse transcription. The extraction method described here does not require ultracentrifugation, and it is fast, simple, and effective. The procedure can be completed within 3 hours and may be applicable to other aromatic medicinal plants containing high amounts of phenolic compounds.