Alterations in growth related genes (GH-I, IGF-I and IGF-II) expression with acute copper exposure in rainbow trout


Journal of Animal and Veterinary Advances, vol.10, no.25, pp.3334-3339, 2011 (SCI-Expanded) identifier

  • Publication Type: Article / Article
  • Volume: 10 Issue: 25
  • Publication Date: 2011
  • Doi Number: 10.3923/javaa.2011.3334.3339
  • Journal Name: Journal of Animal and Veterinary Advances
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.3334-3339
  • Keywords: Copper, Gene expression, Growth hormone, Insulin like growth factors, Multiplex real-time PCR, Rainbow trout
  • Anadolu University Affiliated: Yes


In this study, the acute effects of copper (CuS04.5H20) on the expression of Growth Hormone-I (GH-I) and Insulin like Growth Factors I and II (IGF-I and IGF-II) were investigated in rainbow trout (Oncorhynchus mykiss) muscle tissue. For this purpose, rainbow trout were exposed to 100 jag L-1 (Sublethal concentration) copper sulfate in moderately hard freshwater. After 6, 12, 24 and 48 h of treatment, expression of white muscle GH-I, IGF-I and IGF-II mRNAs were measured by means of quantitative real-time PCR. During the exposure experiments, no mortalities occurred. All mRNA levels significantly decreased after copper administration (p<0.05). The results indicate that short term copper exposure suppresses the mRNA expression levels of GH-I, IGF-I and IGF-II in rainbow trout muscles. © Medwell Journals, 2011.