Proteinous Polymeric Shell Decorated Nanocrystals for the Recognition of Immunoglobulin M

BÜYÜKTİRYAKİ S., Yilmaz F., Say R., ERSÖZ A.

JOURNAL OF FLUORESCENCE, cilt.29, sa.3, ss.609-617, 2019 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 29 Sayı: 3
  • Basım Tarihi: 2019
  • Doi Numarası: 10.1007/s10895-019-02373-5
  • Dergi Adı: JOURNAL OF FLUORESCENCE
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.609-617
  • Anahtar Kelimeler: Orientated conjugation, Recognition of IgM, ANADOLUCA method, Quantum dots, Photosensitive crosslinking, SURFACE-PLASMON RESONANCE, CONCANAVALIN-A, QUANTUM DOTS, DENGUE VIRUS, ANTIBODIES, BINDING, DUPLEXES, LECTIN, PROBE, ASSAY
  • Anadolu Üniversitesi Adresli: Evet

Özet

This study demonstrates the preparation of photosensitively orientated and crosslinked proteinous polymeric shell having quantum dot based nanocrystals through Amino acid Decorated and Light Underpinning Conjugation Approach (ANADOLUCA). ANADOLUCA is based on photo-electron transfer method and uses these decorated nanocrystals for specifically and effectively recognition and detection of Immunoglobulin M in the aqueous environment. The conjugation method effectively provides an orientation of affinity pairs on the surface of quantum dots nanocrystals. This photosensitive ruthenium-based amino acid monomer is a synthetic and inexpensive material for the preparation of bioconjugates. The nanocrystals give advantages for using a wide pH and temperature range. The construction and preparation method is applicable to silica materials, superparamagnetic particles, quantum dots, carbon nanotubes, Ag/Au nanoparticles, Au surfaces, and polymeric materials. This prepared proteinous polymeric shell decorated nanocrystals are of great potential in applications in life sciences and can be used in infection case studies or allergy symptoms.