Synthesis and characterization of 2-aminoethylphosphonic acid-functionalized graphene quantum dots: biological activity, antioxidant activity and cell viability

Yapar G., ŞENEL B., DEMİR N., Yildiz M.

INDIAN JOURNAL OF CHEMISTRY SECTION A-INORGANIC BIO-INORGANIC PHYSICAL THEORETICAL & ANALYTICAL CHEMISTRY, cilt.59, sa.3, ss.317-323, 2020 (SCI-Expanded) identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 59 Sayı: 3
  • Basım Tarihi: 2020
  • Dergi Adı: INDIAN JOURNAL OF CHEMISTRY SECTION A-INORGANIC BIO-INORGANIC PHYSICAL THEORETICAL & ANALYTICAL CHEMISTRY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier
  • Sayfa Sayıları: ss.317-323
  • Anahtar Kelimeler: N-doped graphene quantum dots, Biological activity, Antioxidant activity, MTT, Cell viability, DNA-BINDING, ANION SENSOR, DELIVERY, PROBE, OXIDE
  • Anadolu Üniversitesi Adresli: Evet

Özet

A facile, environmentally friendly one-step reaction for the preparation of luminescent N-doped graphene quantum dots (GQDs) involving a hydrothermal reaction between citric acid and 2-aminoethylphosphonic acid has been designed. Graphene quantum dots have been characterized by UV-visible absorption, FTIR spectroscopy, transmission electron microscopy (TEM), energy dispersive X-ray spectroscopy (EDX) and dynamic light scattering (DLS) techniques. Furthermore, the biological activity of the GQDs has been studied. UV-visible spectroscopy studies of the interactions between the GQDs and calf thymus DNA (CT-DNA) showed that the compound interacts with CT-DNA via intercalative binding. DNA cleavage study showed that the GQDs cleaved DNA oxidatively. In addition, antioxidant activity of N-doped GQDs was measured using the DPPH method. As the concentration of the compound increased, the antioxidant activity also has increased. According to cell viability analyses results, the N-doped GQDs showed cell viability (70%) when the concentration reaches 228 mu g/mL for A549, 200 mu g/mL for MDA-MB-231 and 140 mu g/mL for NIH-3T3 cell lines with 24 h incubation.