Development of immunomagnetic separation technique for isolation of Pseudomonas syringae pv. phaseolicola

Guven K., Mutlu M.

FOLIA MICROBIOLOGICA, cilt.45, sa.4, ss.321-324, 2000 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 45 Sayı: 4
  • Basım Tarihi: 2000
  • Doi Numarası: 10.1007/bf02817554
  • Dergi Adı: FOLIA MICROBIOLOGICA
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.321-324
  • Anadolu Üniversitesi Adresli: Hayır

Özet

Immunomagnetic separation technique was developed for specific detection and selective isolation of Pseudomonas syringae pv, phaseolicola, the agent of halo-blight disease of beans. Whole-cell and exopolysaccharide fraction of the bacterium was used for polyclonal antibody production in rabbits. High specificity of the antisera was determined in agglutination reactions. The optimum immunocapture time for both antisera was determined as 1 h by using 1/nL. CFU (i.e. 10(6) CFU per mt). No significant difference was observed in the binding capacity of cells to immunomagnetic particles with different antisera.