In vitro Hepatotoxicity Evaluation of Atypical Antipscyhotics

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Atlı Eklioğlu Ö.

International Journal of Advances in Science, Engineering and Technology, vol.6, no.4, pp.15-19, 2019 (Peer-Reviewed Journal)


 Liver is the most important target of drugs so that it is very important to determine the hepatotoxicity. HepG2 cells, which are well characterized human hepatoma cells, are the most commonly preferred model for hepatotoxicity evaluation in vitro. It is known that Quetiapine, Olanzapine, Risperidone and Aripiprazole treatment, which are administrated as the top prescribed medications all over the world, has rarely been associated with hepatotoxicity, however some cases of acute liver injury secondary to the use of these antipsychotics were described. Therefore, in this study, we aimed to evaluate hepatotoxicity associated with atypical antipsychotics by using HepG2, human hepatocarcinoma cell line, via cytotoxicity testing and since it is now known that apoptosis in the liver plays a role in the hepatotoxicity of chemicals, flow cytometry was used to detect apoptosis. According to our results, Aripiprazole was found to be the most hepatotoxic drug among the others, and all of the drugs tested were shown to induce cell death by triggering apoptosis. Index Terms - Aripiprazole, in vitro hepatotoxicity testing, flow cytometry, HepG2 cells, olanzapine, risperidone, quetiapine.