Characterization of volatile constituents of Scaligeria tripartita and studies on the antifungal activity against phytopathogenic fungi

Tabanca N., Demirci B., Baser K. H. C., Mincsovics E., Khan S. I., Jacob M. R., ...More

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, vol.850, no.1-2, pp.221-229, 2007 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 850 Issue: 1-2
  • Publication Date: 2007
  • Doi Number: 10.1016/j.jchromb.2006.11.041
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.221-229
  • Keywords: Scaligeria tripartita, Pimpinella species, overpressured layer chromatography (OPLC), essential oil, GCiMS, Apiaceae, phenylpropanoids, antimalarial activity, antimicrobial activity, OVERPRESSURED-LAYER CHROMATOGRAPHY, FLOWING ELUENT WALL, ANTIMICROBIAL COMPOUNDS, MASS SPECTROMETRY, CANARY-ISLANDS, ESSENTIAL OILS, AERIAL PARTS, PIMPINELLA, OPLC, SEPARATION
  • Anadolu University Affiliated: Yes


The chemical composition of the essential oils obtained from stems and leaves, fruits and roots of Scaligeria tripartita oil was analyzed by gas chromatography-mass spectrometry. A total of 38 compounds were identified ranging 89-94% of the oil samples. Geijerene was found as a main compound in the oils of the stems and leaves (37%) and fruits (55%), whereas epoxypseudoisoeugenol angetate (37%) was found as a main compound in the root oil. Oils were subsequently evaluated for their antimalarial, antimicrobial against human pathogenic bacteria or fungi and antifungal activities against plant pathogens. Antifungal activity of Scaligeria oils was observed against the strawberry anthracnose-causing fungal plant pathogens Colletotrichum acutatum, C. fragariae and C. gloeosporioides using the direct overlay bioautography assay. Chemotaxonomically important pure compounds indicated in the bioautography assay were subsequently evaluated in a 96-well microdilution broth assay. The performance of overpressured layer chromatography (OPLC) and TLC for the analysis of Scaligeria essential oils was also compared. (c) 2006 Elsevier B.V. All rights reserved.