Porous poly(hydroxyethyl methacrylate) based monolith as a new adsorbent for affinity chromatography

UZUN L., Say R., Denizli A.

REACTIVE & FUNCTIONAL POLYMERS, vol.64, no.2, pp.93-102, 2005 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 64 Issue: 2
  • Publication Date: 2005
  • Doi Number: 10.1016/j.reactfunctpolym.2005.05.003
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.93-102
  • Keywords: monoliths, antibody separation, protein purification, pseudospecific adsorbents, IgG, IMMUNOGLOBULIN-G, PROTEIN-A, MONOCLONAL-ANTIBODIES, STEP PURIFICATION, STATIONARY PHASES, L-HISTIDINE, SEPARATION, EFFICIENT, SORPTION, DESIGN
  • Anadolu University Affiliated: No


A new approach was developed to obtain an efficient and cost effective purification of IgG from human plasma. Porous monoliths were obtained by the bulk polymerization of 2-hydroxyethyl methacrylate (HEMA) and N-methacryloyl(L)-histidinemethylester (MAH) conducted in a glass tube. Poly(HEMA-MAH) monolith had a specific surface area of 145.8 m(2)/g. Poly(HEMA-MAH) monolith was characterized by swelling studies, FTIR, scanning electron microscopy and elemental analysis. Poly(HEMA-MAH) monolith with a swelling ratio of 68%, and containing 47.8 mu mol MAH/g were used in the adsorption/desorption of IgG from aqueous solutions and human plasma. The maximum amount of IgG adsorption from aqueous solution in phosphate buffer was 9.8 mg/g at pH 7.4. A higher adsorption value was obtained from human plasma (up to 96.5 mg/g) with a purity of 95.3%. It was observed that IgG could be repeatedly adsorbed and desorbed with Poly(HEMA-MAH) monolith without significant loss in the adsorption capacity. (c) 2005 Elsevier B.V. All rights reserved.