© 2020 Bulgarian Academy of Sciences, Union of Chemists in BulgariaRosa damascena Mill. is a traditional and promising agricultural plant used for manufacturing essential oil in Saudi Arabia. The present study aims at studying the chemical composition, sensory evaluation and antimicrobial activity of some samples of rose oils (Rosa damascena Mill.) from the region of Taif. The chemical composition was determined by GC and GC/MS. Main constituents in the studied essential oils were: citronellol, nerol, geraniol, and nonadecane. The monoterpene alcohols, citronellol, geraniol, and nerol, were the constituents responsible for the odor of the oils analysed. The antimicrobial activity was determined against Gram-positive bacteria (Listeria monocytogenes NCTC 11994, Staphylococcus aureus ATCC 25093, and Bacillus cereus ATCC 11778), Gram-negative bacteria (Escherichia coli ATCC 8739 and Salmonella enterica subsp. enterica serovar Abony NCTC 6017), and a fungal strain - Aspergillus flavus. The high antimicrobial effect of these rose oils was confirmed on six types of bacteria and fungi. The strongest antimicrobial effect was against the Gram-positive bacterium S. aureus. The specified characteristics of Taif rose oils indicate their potential implementation in perfumery, cosmetic, soap, household and personal care products manufactured in the Kingdom of Saudi Arabia.