© 2020, Badebio Biotechnololgy Ltd. All rights reserved.Chemical composition of the essential oils obtained by hydrodistillation from the aerial parts of Anthemis cretica subsp. anatolica was analyzed by gas chromatography (GC) and gas chromatography-mass spectroscopy (GC-MS) systems. The essential oil of A. cretica subsp. anatolica was characterized by the presence of a high percentage of oxygenated sesquiterpenes (57.9%). Twenty-seven compounds were identified representing 96.6 % of the essential oil of A. cretica subsp. anatolica. The main components of the oil were spathulenol (27.0%) and hexadecanoic acid (14.3%).