Design, synthesis, monoamine oxidase inhibition and docking studies of new dithiocarbamate derivatives bearing benzylamine moiety


KAYA ÇAVUŞOĞLU B., SAĞLIK B. N., ÖZKAY Y., Inci B., KAPLANCIKLI Z. A.

BIOORGANIC CHEMISTRY, vol.76, pp.177-187, 2018 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 76
  • Publication Date: 2018
  • Doi Number: 10.1016/j.bioorg.2017.11.012
  • Journal Name: BIOORGANIC CHEMISTRY
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Index Chemicus (IC)
  • Page Numbers: pp.177-187
  • Keywords: Human monoamine oxidase, Arylalkyl amine moiety, Amplex red, Molecular docking, CLINICAL-PHARMACOLOGY, BIOLOGICAL EVALUATION, PERMEABILITY, SOLUBILITY, PREDICTION, HYDRAZONE, POTENT
  • Anadolu University Affiliated: Yes

Abstract

A new series of thirteen 2-[(4-fluorophenyl)(4-nitrobenzyl) amino]-2-oxoethyl-1-substituted-carbodithio ate derivatives (4a-4m) were synthesized and tested for their human monoamine oxidase A and B (hMAO-A and hMAO-B) inhibitory potential by an in vitro fluorometric method. Most of the compounds have found to be selective towards MAO-B than MAO-A. Compound 4j that carrying 4-nitrophenyl piperazine moiety, was detected as the most active agent amongst all compounds with the IC50 value of 0.097 +/- 0.003 mu M for MAO-B while that of selegiline was 0.040 +/- 0.002 mu M. The enzyme kinetic study reported that compound 4j is a reversible and non-competitive inhibitor. Interaction modes between the hMAO-B and compound 4j were determined by docking studies. The study also revealed that compound 4j has the highest binding scores. Besides, compound 4j has not cytotoxicity at its effective concentration against hMAO-B. (C) 2017 Elsevier Inc. All rights reserved.