Isolation of natural products by preparative gas chromatography

ÖZEK T., DEMİRCİ F.

Methods in Molecular Biology, vol.864, pp.275-300, 2012 (Scopus) identifier identifier

  • Publication Type: Article / Article
  • Volume: 864
  • Publication Date: 2012
  • Doi Number: 10.1007/978-1-61779-624-1_11
  • Journal Name: Methods in Molecular Biology
  • Journal Indexes: Scopus
  • Page Numbers: pp.275-300
  • Keywords: Chromatography, Fractionation, GC, GC-MS, Isolation, Preparative separation, Terpenes, Volatiles
  • Anadolu University Affiliated: Yes

Abstract

Chromatography is a process for the separation of compounds from the mixtures. Gas chromatography (GC) is one of the most important and common techniques in separation science. The main focus of this chapter is to demonstrate a hands-on approach to preparative gas chromatography (Prep-GC) technique and its use for the isolation of natural products, particularly terpenes and other volatiles. Starting from the classification of GC separations, columns, and stationary phases, sampling techniques, mobile phases, detectors, and fraction collector systems of analytical and preparative gas chromatographic separations are comparatively covered in this chapter. © 2012 Springer Science+Business Media, LLC.