Nanosensors having dipicolinic acid imprinted nanoshell for Bacillus cereus spores detection

Gultekin A., ERSÖZ A., YILMAZ SARIÖZLÜ N., DENİZLİ A., Say R.

JOURNAL OF NANOPARTICLE RESEARCH, vol.12, no.6, pp.2069-2079, 2010 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 12 Issue: 6
  • Publication Date: 2010
  • Doi Number: 10.1007/s11051-009-9766-z
  • Journal Name: JOURNAL OF NANOPARTICLE RESEARCH
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.2069-2079
  • Keywords: Gold-silver nanoclusters sensor, Molecularly imprinted polymers, Dipicolinic acid, Bacillus cereus spores recognition, Photoluminescence, ENHANCED RAMAN-SPECTROSCOPY, SURFACE-PLASMON RESONANCE, ENDOSPORE DETECTION, THERMAL-RESISTANCE, ANTHRACIS SPORES, PHOTOLUMINESCENCE, NANOPARTICLES, POLYMER, AU, DEACTIVATION
  • Anadolu University Affiliated: Yes

Abstract

Molecular imprinted polymers (MIPs) as a recognition element for sensors are increasingly of interest and MIP nanoclusters have started to appear in the literature. In this study, we have proposed a novel thiol ligand-capping method with polymerizable methacryloylamido-cysteine (MAC) attached to gold-silver nanoclusters, reminiscent of a self-assembled monolayer and have reconstructed surface shell by synthetic host polymers based on molecular imprinting method for recognition. In this method, methacryloylamidoantipyrine-terbium ((MAAP)(2)-Tb(III)) has been used as a new metal-chelating monomer via metal coordination-chelation interactions and dipicolinic acid (DPA) which is main participant of Bacillus cereus spores used as a model. Nanoshell sensors with templates give a cavity that is selective for DPA. The DPA can simultaneously chelate to Tb(III) metal ion and fit into the shape-selective cavity. Thus, the interaction between Tb(III) ion and free coordination spheres has an effect on the binding ability of the gold-silver nanoclusters nanosensor. The binding affinity of the DPA imprinted nanoclusters has been investigated by using the Langmuir and Scatchard methods, and the respective affinity constants (K (affinity)) determined were found to be 1.43 x 10(4) and 9.1 x 10(6) mol L(-1).