Synthesis, characterization of some transition-metal complexes of a new heptadentate N5S2 Schiff-base ligand and the effects of these metal complexes on U2OS cells cytotoxicity and DNA cleavage activity
PHOSPHORUS SULFUR AND SILICON AND THE RELATED ELEMENTS, cilt.183, sa.9, ss.2237-2247, 2008 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 183 Sayı: 9
- Basım Tarihi: 2008
- Doi Numarası: 10.1080/10426500801907589
- Dergi Adı: PHOSPHORUS SULFUR AND SILICON AND THE RELATED ELEMENTS
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
- Sayfa Sayıları: ss.2237-2247
- Anahtar Kelimeler: mitocondrial activity, MTT, Schiff base, template synthesis, CRYSTAL-STRUCTURES, THIOSEMICARBAZONE, COORDINATION, IONS
- Anadolu Üniversitesi Adresli: Evet
Özet
Metal complexes of a heptadentate N5S2 donor Schiff-base ligand were synthesized by the template reaction between 2,6-bis(2-aminothiophenoxymethyl)pyridine and 2,2 '-bipyridine-6,6'-dicarboxaldehyde in the presence of Zn(II), Cd(II), Hg(II) and Pb(II) perchlorate salts. The complexes were characterized by IR, 1H NMR, MS-FAB+, and elemental analyses. The mitochondrial activity of U2OS cells after exposure to these complexes was determined by colorimetric assay, which detects the conversion of 3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromide (MTT) to formazon. DNA-binding activity of all complexes was also investigated using plasmid DNA pUC18 purified from E. coli.