Synthesis, characterization of some transition-metal complexes of a new heptadentate N5S2 Schiff-base ligand and the effects of these metal complexes on U2OS cells cytotoxicity and DNA cleavage activity

Ulucam G., Beynek N., Seller Z., Akalin G., Turan G., Benkli K.

PHOSPHORUS SULFUR AND SILICON AND THE RELATED ELEMENTS, vol.183, no.9, pp.2237-2247, 2008 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 183 Issue: 9
  • Publication Date: 2008
  • Doi Number: 10.1080/10426500801907589
  • Journal Name: PHOSPHORUS SULFUR AND SILICON AND THE RELATED ELEMENTS
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.2237-2247
  • Keywords: mitocondrial activity, MTT, Schiff base, template synthesis, CRYSTAL-STRUCTURES, THIOSEMICARBAZONE, COORDINATION, IONS
  • Anadolu University Affiliated: Yes

Abstract

Metal complexes of a heptadentate N5S2 donor Schiff-base ligand were synthesized by the template reaction between 2,6-bis(2-aminothiophenoxymethyl)pyridine and 2,2 '-bipyridine-6,6'-dicarboxaldehyde in the presence of Zn(II), Cd(II), Hg(II) and Pb(II) perchlorate salts. The complexes were characterized by IR, 1H NMR, MS-FAB+, and elemental analyses. The mitochondrial activity of U2OS cells after exposure to these complexes was determined by colorimetric assay, which detects the conversion of 3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromide (MTT) to formazon. DNA-binding activity of all complexes was also investigated using plasmid DNA pUC18 purified from E. coli.