Passage of vasoactive intestinal peptide across the blood-brain barrier

Dogrukol-Ak D., Banks W., Tuncel N., Tuncel M.

PEPTIDES, no.3, pp.437-444, 2003 (SCI-Expanded) identifier identifier identifier identifier

  • Publication Type: Article / Article
  • Publication Date: 2003
  • Doi Number: 10.1016/s0196-9781(03)00059-7
  • Journal Name: PEPTIDES
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.437-444
  • Keywords: vasoactive intestinal peptide, blood-brain barrier, high-performance liquid chromatography, unidirectional influx rate, cerebrospinal fluid, intracerebroventricular injection, CYCLASE-ACTIVATING POLYPEPTIDE, ISCHEMIA-REPERFUSION, CELL-DEATH, TNF-ALPHA, VIP, TRANSPORT, RATS, SURVIVAL, MICE, QUANTIFICATION
  • Anadolu University Affiliated: Yes

Abstract

We investigated the ability of vasoactive intestinal peptide (VIP) to cross the blood-brain barrier (BBB), the interface between the peripheral circulation and central nervous system (CNS). VIP labeled with I-131 (I-VIP) and injected intravenously into mice was taken up by brain as determined by multiple-time regression analysis. Excess unlabeled VIP was unable to impede the entry of I-VIP, indicating that passage is by nonsaturable transmembrane diffusion. High pressure liquid chromatography (HPLC) showed the radioactivity entering the brain to be intact I-VIP. After intracerebroventricular (i.c.v.) injection, I-VIP was sequestered by brain, slowing its efflux from the CNS. In summary, VIP crosses the BBB unidirectionally from blood to brain by transmembrane diffusion. (C) 2003 Elsevier Science Inc. All rights reserved.