The in vitro assessment of dipyridophenazine complexes in H-ras oncogene transformed rat embryo fibroblast 5RP7 cell line

Kaplan A., Benkli K., Koparal A. T.

INVESTIGATIONAL NEW DRUGS, cilt.36, sa.5, ss.755-762, 2018 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 36 Sayı: 5
  • Basım Tarihi: 2018
  • Doi Numarası: 10.1007/s10637-017-0559-4
  • Dergi Adı: INVESTIGATIONAL NEW DRUGS
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.755-762
  • Anahtar Kelimeler: Dipyridophenazine, Platinum(II), Gold(III), Cytotoxicity, Apoptosis, DAPI, DNA-BINDING PROPERTIES, PLATINUM(II) COMPLEXES, POLYPYRIDYL COMPLEXES, ANTICANCER ACTIVITY, ANTITUMOR AGENTS, CYTOTOXICITY, DERIVATIVES, CHEMISTRY, INHIBITORS, TOXICITY
  • Anadolu Üniversitesi Adresli: Evet

Özet

Purpose The aim of this study is to detect apoptotic and cytotoxic/antiproliferative effects of a ligand substance and its metal derivatives. The substances were investigated by using an h-ras oncogene transformed rat embryo fibroblast cell line (5RP7). Methods The cytotoxic influences of dipyrido[3,2-a:2,3'c]phenazine ligand, dipyrido[3,2-a:2,3'c] phenazine-platinum(II) complex ([Pt(dppz)Cl-2]) and dipyrido[3,2-a:2,3'c] phenazine-gold(III) complex ([Au(dppz)Cl-2]Cl) were determined with MTT (3[4,5-dimetiltiyazol2-yl]-2,5-difeniltetrazolyum bromid) assay on 5RP7 cells. Results Dipyrido[3,2-a:2,3'c] phenazine, dipyrido[3,2-a:2,3'c] phenazine-platinum(II) complex ([Pt(dppz)Cl-2]) and dipyrido[3,2-a:2,3'c] phenazine-gold(III) complexes ([Au(dppz)Cl-2]Cl) caused significant increase in cytotoxicity in a dose and time dependent manner. The effects of dipyridophenazine ligand (dppz) and its metal derivatives on apoptosis were monitorized using cytotoxic dose (10M) DAPI fluorescent staining. It was shown that dppz and its compounds induced apoptosis. Conclusions These findings show that dpzz and its complexes can be studied as novel alternative chemotherapeutics in cancer treatment.