MgSO4 and lazaroid (U-83836E) partially protects glioma cells against glutamate toxicity in vitro

KABADERE S., Oztopcu P., Korkmaz S., Erol K., Uyar R.

ACTA NEUROBIOLOGIAE EXPERIMENTALIS, cilt.64, sa.4, ss.461-466, 2004 (SCI-Expanded) identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 64 Sayı: 4
  • Basım Tarihi: 2004
  • Dergi Adı: ACTA NEUROBIOLOGIAE EXPERIMENTALIS
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.461-466
  • Anahtar Kelimeler: glutamate toxicity, MgSO4, lazaroid, glioma, GLIAL-CELLS, HEAD-INJURY, MAGNESIUM, NEURONS, BRAIN, MICROCULTURE, NIMODIPINE, SYNAPSES, ISCHEMIA, CULTURES
  • Anadolu Üniversitesi Adresli: Hayır

Özet

In this study, the possible effects Of MgSO4 and lazaroid (U-83836E) on glutamate toxicity on glial cells were investigated. C6 and human glioblastoma multiforme cells derived from two patients were grown in an incubator. First, determined IC50 dose of L-glutamate (L-glu) was given for 24 hours and removed, and then respective MgSO4 or U-83836E doses were added to the culture medium. After 24 hours 3-(4,5-Dimethylthyazol-2-yl)-2,5-diphenyltetrazolium bromide, thiazolyl blue (MTT) test was applied. When compared to the L-glu-treated group, MgSO4 at the dose of 0.01 mM induced C6 and human glioma cell growth by 17%, 15% and 5%, respectively. At the dose of I muM U-83836E also increased C6 and human glioma cell growth by 12%, 13% and 5%, respectively. In conclusion, although MgSO4 and U-83836E do not strongly block glutamate-induced cell death, it is suggested that reduction of Mg2+ ions and free radical production may have a role in glutamate toxicity on glial cells.