In this study, Ce3+-based cryogel with methacryloyl antipyrine (MAAP) and 2-hydroxyethyl methacrylic acid (HEMA) [p(HEMA-MAAP-Ce3+] was prepared. MAAP-Ce3+ complex was characterized by UV-near infrared and energy-dispersive X-ray spectroscopy. Cryogel was characterized by Fourier transform infrared spectroscopy, scanning electron microscopy, and swelling test. Pore size of the cryogel was found to be about 30-50 mu m. The effects of flow rate, pH, temperature, and initial enzyme concentration have been investigated. Maximum adsorption capacity was found to be 57.84 mg g(-1) cryogel at pH 6.0. After seven times of adsorption-desorption cycles of same cryogel, it was observed that there is negligible decrease in the adsorption capacity.