Adsorption behaviours of lysozyme onto poly-hydroxyethyl methacrylate cryogels containing methacryloyl antipyrine-Ce(III)

BAYSAL Z., Aksoy E., DOLAK İ., ERSÖZ A., Say R.

INTERNATIONAL JOURNAL OF POLYMERIC MATERIALS AND POLYMERIC BIOMATERIALS, vol.67, no.4, pp.199-204, 2018 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 67 Issue: 4
  • Publication Date: 2018
  • Doi Number: 10.1080/00914037.2017.1320655
  • Journal Name: INTERNATIONAL JOURNAL OF POLYMERIC MATERIALS AND POLYMERIC BIOMATERIALS
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.199-204
  • Keywords: Adsorption, cerium(3+), cryogel, lanthanide, lysozyme, AFFINITY PURIFICATION, POLYMERIC CRYOGELS, EGG-WHITE, IMMOBILIZATION, CHROMATOGRAPHY, NANOPARTICLES, UREASE, IONS, ACID
  • Anadolu University Affiliated: Yes

Abstract

In this study, Ce3+-based cryogel with methacryloyl antipyrine (MAAP) and 2-hydroxyethyl methacrylic acid (HEMA) [p(HEMA-MAAP-Ce3+] was prepared. MAAP-Ce3+ complex was characterized by UV-near infrared and energy-dispersive X-ray spectroscopy. Cryogel was characterized by Fourier transform infrared spectroscopy, scanning electron microscopy, and swelling test. Pore size of the cryogel was found to be about 30-50 mu m. The effects of flow rate, pH, temperature, and initial enzyme concentration have been investigated. Maximum adsorption capacity was found to be 57.84 mg g(-1) cryogel at pH 6.0. After seven times of adsorption-desorption cycles of same cryogel, it was observed that there is negligible decrease in the adsorption capacity.