HPLC fluorescence determination of ochratoxin A utilizing a double internal standard and its application to poultry feed

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Turkish Journal of Chemistry, vol.39, no.2, pp.372-381, 2015 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 39 Issue: 2
  • Publication Date: 2015
  • Doi Number: 10.3906/kim-1408-49
  • Journal Name: Turkish Journal of Chemistry
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, TR DİZİN (ULAKBİM)
  • Page Numbers: pp.372-381
  • Keywords: Animal feed and poultry feed, fluorescence detector, HPLC, mycotoxin, ochratoxin A, CEREAL-DERIVED PRODUCTS, CAPILLARY-ELECTROPHORESIS, EXPOSURE ASSESSMENT, BLOOD, QUANTIFICATION
  • Anadolu University Affiliated: Yes


© TÜBİTAK.A validated liquid chromatography method employing a fluorescence detector for the determination of ochratoxin A (OTA) was developed with double internal standard and it was applied to ten different poultry feeds. The analysis was performed in an octadecyl silane column using a solvent system [ACN:water:formic acid (50:50:1.25, v/v/v)] by isocratic elution. The flow rate and injection volume were 1 mL min-1 and 12 μL, respectively. Signals were detected at 278(λex) /315(λem) and 330(λex) /450(λem) nm between 0 and 8, and 8.01 and 20.0 min, respectively. The method was validated with precision, linearity, accuracy, limit of detection, limit of quantification, robustness, and stability. Good linearity (r2 = 0.9998-0.9999) was achieved over a concentration range of 1.60 × 10-8 M to 6.40 × 10-6 M for OTA. LOD and LOQ values were 7.83 × 10-10 M and 2.37 × 10-9 M, and 2.01 × 10-9 M and 6.10 × 10-9 M for internal standard 1 (IS1) and internal standard 2 (IS2), respectively, on an interday basis. The method was applied to poultry feed samples. Good recovery data ranged between 79.10% and 85.57%, and 71.98% and 76.66%, and the RSD% values were in the range of 1.36-11.70 and 2.07-2.34 for IS1 and IS2, respectively.