Synthesis and characterization of Bodipy functionalized magnetic iron oxide nanoparticles for potential bioimaging applications

Topel S. D., TOPEL Ö., Bostancioğlu R. B., KOPARAL A. T.

Colloids and Surfaces B: Biointerfaces, vol.128, pp.245-253, 2015 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 128
  • Publication Date: 2015
  • Doi Number: 10.1016/j.colsurfb.2015.01.043
  • Journal Name: Colloids and Surfaces B: Biointerfaces
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.245-253
  • Keywords: Bio-imaging, Bodipy, Cytotoxicity, Magnetic nanoparticles, Multifunctional nanoparticles
  • Anadolu University Affiliated: Yes

Abstract

Multifunctional magnetic nanoparticles were synthesized for potential bio-imaging applications. Uniform PEI coated magnetic Fe3O4(PEI-Fe3O4) nanoparticles were prepared by a modified co-precipitation method and then covalently conjugated with a fluorophore molecule, Bodipy-5 by the DCC/DMAP coupling reaction. The covalent binding of Bodipy-5 to the PEI coated magnetic Fe3O4nanoparticles were confirmed by means of FTIR and XPS measurements. The imaging ability of the Bodipy coated magnetic nanoparticles was determined on two human cancer cells, A549 (human lung adenocarcinoma epithelial) and Ishikawa (endometrial adenocarcinoma), for the first time. Cytotoxicity of BOD-MNPs was evaluated in both cancer cells and healthy human umbilical vein endothelial cell line (HUVEC) by standard MTT (3-(4,5-dimethythiazol-2-yl)-2,5-diphenyl tetrazolium bromide) assay. In vitro activities of the nanoparticles were also investigated.