Diversity, characterization and antimicrobial activities of Bacillus cereus strains isolated from soil

GÜVEN K., İLHAN S., MUTLU M. B., ÇOLAK F.

FRESENIUS ENVIRONMENTAL BULLETIN, vol.17, no.3, pp.303-310, 2008 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 17 Issue: 3
  • Publication Date: 2008
  • Journal Name: FRESENIUS ENVIRONMENTAL BULLETIN
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.303-310
  • Keywords: Bacillus cereus, antimicrobial activity, FAME, ribotyping, BCET-RPLA, PROTEIN-PATTERNS, GENUS BACILLUS, FATTY-ACIDS, RESISTANCE, INDUSTRIAL, ZWITTERMICIN, ENUMERATION, BACTERIOCIN, PREVALENCE, RANGE
  • Anadolu University Affiliated: Yes

Abstract

In this study, 10 Bacillus cereus strains were isolated from different soil samples. Selective isolation of the strains was carried out on MYP agar medium. Biochemical identification was also confirmed by fatty acid methyl ester (FAME) analysis and ribotyping. Strains were analysed phenotypically by sensitivity to common antibiotics, diarrhoeal enterotoxin production by BCET-RPLA, SDS-PAGE analyses of whole cell proteins. Genotyping included automatized ribotyping by Eco RI enzyme.