Akademik Veri Yönetim Sistemi
X. Multidisciplinary Cancer Research Congress , Eskişehir, Türkiye, 8 - 11 Mayıs 2025, ss.99, (Özet Bildiri)
Introduction and Aim: Non-small cell lung cancer (NSCLC) is the most common subtype, and chemotherapy resistance remains a major challenge. Cisplatin is widely used, while resveratrol, a natural polyphenol, may enhance chemotherapy efficacy and reduce side effects. This study explores the effects of resveratrol and cisplatin, both alone and in combination, on A549(lung cancer) and A549/CDDP(cisplatin-resistant) cell lines. Materials and Methods: The cytotoxic, apoptotic and autophagic effects of resveratrol and cisplatin were assessed via MTT assay, Annexin V/PI flow cytometry, Autophagic vesicles visualized by fluorescent labeling. Results: After 48 hours of incubation, the IC50 doses of cisplatin and resveratrol were 24.91µM and 122.5µM in A549, 51.52µM and 100µM in A549/CDDP, and 21.2µM and 154.7µM in BEAS-2B (healthy lung) cells. Resveratrol showed a stronger effect on resistant cell lines than on A549 cells, with a lower effect on healthy BEAS-2B cells. Combination ratios of 10%, 20%, 30%, 40%, 50%, 75% and 100% of the IC50 doses of cisplatin and resveratrol were prepared and their combination indices were calculated. The results indicated an antagonistic effect at 10% and a synergistic effect at 40% and 50%, leading to the selection of these ratios for further evaluation of their apoptotic effects. In A549/CDDP cells, apoptosis rates were 16.79%(cisplatin), 16.52%(resveratrol), and 5.15%, 11.53%, and 21.1% for 10%, 40%, and 50% combinations, respectively. In A549 cells, apoptosis rates were 20.44%(cisplatin), 15.29%(resveratrol), and 10.77%, 14.8%, and 17.84% for the same combinations. Apoptosis was higher at the synergistic combination ratios of 40% and 50%. While autophagic vesicle signal intensity decreased in the A549 cell line, no significant difference in signal intensity was observed in the A549/CDDP cell line. Discussion: In conclusion, resveratrol and cisplatin showed a synergistic apoptotic effect at 40% and 50% doses, with selective cytotoxicity in resistant cells. Autophagic activity decreased in A549 cells but remained unchanged in A549/CDDP cells.