Calmodulin content and in vitro contractility of duodenum from streptozotocin-induced diabetic rats: Effects of insulin therapy and calmodulin antagonism
EUROPEAN JOURNAL OF PHARMACOLOGY, cilt.321, sa.1, ss.59-65, 1997 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 321 Sayı: 1
- Basım Tarihi: 1997
- Doi Numarası: 10.1016/s0014-2999(96)00928-4
- Dergi Adı: EUROPEAN JOURNAL OF PHARMACOLOGY
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
- Sayfa Sayıları: ss.59-65
- Anahtar Kelimeler: diabetic rat, streptozotocin, Ca2+, calmodulin, calmidazolium, insulin, intestinal reactivity, SMOOTH-MUSCLE CONTRACTION, BETA-ADRENERGIC RESPONSES, CALCIUM-BINDING PROTEINS, VASCULAR REACTIVITY, GASTROINTESTINAL-TRACT, LIGHT-CHAIN, MELLITUS, MANAGEMENT, CALPONIN, HYPERREACTIVITY
- Anadolu Üniversitesi Adresli: Evet
Özet
The effects of experimental diabetes and insulin treatment on the decreased reactivity of isolated rat duodenum to KCI and calmidazolium, a specific calmodulin antagonist, were examined. After 8 weeks of streptozotocin diabetes, the contractile effect of KCI and the non-competitive antagonistic effect of calmidazolium against KCI on isolated rat duodenum were decreased. Calmodulin levels, as measured by radioimmunoassay, were also found to be decreased in duodenum from streptozotocin-diabetic rats. Neither impaired reactivity to KCI nor decreased calmodulin levels in diabetic rat duodenum were corrected by treatment with insulin (10 IU/kg for 20 days). Following insulin treatment, there was only a partial correction in the antagonistic effect of calmidazolium as shown by the increase in non-competitive antagonist affinity constant.