Simultaneous depletion of albumin and immunoglobulin G by using twin affinity magnetic nanotraps

BÜYÜKTİRYAKİ S., UZUN L., DENİZLİ A., Say R., ERSÖZ A.

SEPARATION SCIENCE AND TECHNOLOGY, cilt.51, sa.12, ss.2080-2089, 2016 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 51 Sayı: 12
  • Basım Tarihi: 2016
  • Doi Numarası: 10.1080/01496395.2016.1200086
  • Dergi Adı: SEPARATION SCIENCE AND TECHNOLOGY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.2080-2089
  • Anahtar Kelimeler: Albumin depletion, IgG depletion, dye-ligand affinity chromatography, protein a affinity chromatography, covalent conjugation, HUMAN SERUM, CIBACRON BLUE, INTERACTION CHROMATOGRAPHY, MASS-SPECTROMETRY, STATIONARY-PHASE, PROTEIN-G, ADSORPTION, DYE, SEPARATION, PLASMA
  • Anadolu Üniversitesi Adresli: Evet

Özet

For biomarker discovery, simultaneous removal of albumin and immunoglobulin G (IgG) become more important for both concentrating other species including potential biomarkers and getting rid of their masking effect. In this study, we have proposed a covalent and photosensitive cross-linking conjugation of the biomolecules on nanostructures for the depletion of target proteins from aqueous solution and serum. The effect of concentration, pH, temperature and ionic strength on the adsorption of twin affinity nanotraps based on Cibacron Blue F3GA and Protein A were investigated. The efficiency of albumin and IgG depletion from human serum was performed using sodium dodecylsulfate-polyacrylamide gel electrophoresis.