Evaluation of the relationship among gene expressions and enzyme activities with antioxidant role and presenilin 1 expression in Alzheimer's disease

IŞIK M., Tunc A., Duran H. E., Naldan M. E., Yilmaz A., KOÇAK M. N., ...More

JOURNAL OF CELLULAR AND MOLECULAR MEDICINE, vol.27, no.21, pp.3388-3394, 2023 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 27 Issue: 21
  • Publication Date: 2023
  • Doi Number: 10.1111/jcmm.17953
  • Journal Name: JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, BIOSIS, MEDLINE, Directory of Open Access Journals
  • Page Numbers: pp.3388-3394
  • Keywords: Alzheimer's disease, antioxidant status, enzyme activity, gene expression, paraoxonase 1, presenilin 1
  • Anadolu University Affiliated: Yes

Abstract

It is known that oxidative stress originating from reactive oxygen species plays a role in the pathogenesis of Alzheimer's disease. In this study, the role of antioxidant status associated with oxidative stress in Alzheimer's disease was investigated. Peripheral blood samples were obtained from 28 healthy individuals (as control) and 28 Alzheimer's patients who met the National Institute of Neurological and Communicative Diseases and Stroke/Alzheimer's Disease and Related Disorders Association criteria. Catalase, glutathione S-transferase and paraoxonase 1 enzyme activities in blood plasma and glutathione S-transferase enzyme activities in erythrocytes were determined by spectrophotometer. Catalase, glutathione S-transferase and presenilin 1 gene expressions in leukocytes were determined using qRT-PCR. Data were analysed with SPSS one-way anova, a LSD post hoc test at p < 0.05. The activity of each enzyme was significantly reduced in Alzheimer's patients compared to control. The catalase gene expression level did not change compared to the control. Glutathione S-transferase and presenilin 1 gene expression levels were increased compared to the control.