Comparison of adsorption performances of metal-chelated polyamide hollow fibre membranes in lysozyme separation

Senel S., Kassab A., Arica Y., Say R., Denizli A.

COLLOIDS AND SURFACES B-BIOINTERFACES, cilt.24, sa.3-4, ss.265-275, 2002 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 24 Sayı: 3-4
  • Basım Tarihi: 2002
  • Doi Numarası: 10.1016/s0927-7765(01)00278-8
  • Dergi Adı: COLLOIDS AND SURFACES B-BIOINTERFACES
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.265-275
  • Anahtar Kelimeler: polyamide hollow fibres, dye-chelate chromatography, lysozyme, Cibacron Blue F3GA, HUMAN-IMMUNOGLOBULIN-G, AFFINITY MEMBRANES, HUMAN PLASMA, ALBUMIN ADSORPTION, PROTEIN ADSORPTION, FIBER MEMBRANES, TRIAZINE DYES, PURIFICATION, CHROMATOGRAPHY, ADSORBENTS
  • Anadolu Üniversitesi Adresli: Hayır

Özet

Commercially available microporous polyamide hollow fibres are modified by acid hydrolysis to activate the reactive groups and subsequently binding of the ligand, i.e. Cibacron Blue F3GA. Then the Cibacron Blue F3GA-derived hollow fibres were loaded with different metal ions (i.e. Zn(II), Cu(II), Ni(II)) to form the metal chelate. The internal polymer matrix was characterised by scanning electron microscopy. The effects of pH, initial concentration of lysozyme, metal type and temperature on the adsorption of lysozyme to the metal-chelated hollow fibres were examined in a batch reactor. The non-specific adsorption of lysozyme onto the polyamide hollow fibres was 1.8 mg/g. Cibacron Blue F3GA immobilisation increased the lysozyme adsorption up to 62.3 mg/g. Metal-chelated hollow fibres showed a significant increase of the adsorption efficiency. Lysozyme adsorption capacities of Zn(II), Cu(II) and Ni(Il)-chelated hollow fibres were different. The maximum capacities of Zn(II), Cu(II) or Ni(Il)-chelated hollow fibres were 144.2, 75.2 and 68.6 mg/g, respectively. Significant amount of the adsorbed lysozyme (up to 97%) was eluted in 1 h in the elution medium containing 1.0 M NaSCN at pH 8.0 and 25 mM EDTA at pH 4.9. Repeated adsorption-desorption process showed that this novel metal-chelated polyamide hollow fibres are suitable for lysozyme adsorption. (C) 2002 Published by Elsevier Science B.V.