ULTRASTRUCTURAL EXAMINATION OF INTRACELLULAR CALCIUM CHANGES IN OVARIAN CANCER

Ayriml A., DAĞ İ., Incesu Z.

FRESENIUS ENVIRONMENTAL BULLETIN, vol.26, no.11, pp.6588-6598, 2017 (SCI-Expanded) identifier

  • Publication Type: Article / Article
  • Volume: 26 Issue: 11
  • Publication Date: 2017
  • Journal Name: FRESENIUS ENVIRONMENTAL BULLETIN
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED)
  • Page Numbers: pp.6588-6598
  • Keywords: Ovarian cancer, Integrin, Calcium, Electron Microscope, Apoptosis, CELLS, APOPTOSIS, INHIBITION, AUTOPHAGY, INTEGRINS
  • Anadolu University Affiliated: Yes

Abstract

In this study, following the fibronectin-coated integrin activation in ovarian cancer cells (SKOV-3), endoplasmic reticulum (ER) stress was generated by intracellular calcium increase and then possible apoptotic effects were investigated. Tunicamycin (TN) concentrations and intracellular calcium alterations in SKOV-3 cells were analyzed by flow cytometry using Fluo-3 AM ye Rhod-2 AM flou-rescent dyes. Apoptotic cell death dependent to ER stress that generated after the treatment with 18 mu M TN were investigated by Annexin V / PI protocol and the localisations of cytochrome c and EndoG proteins were also imaged by fluorescent microscope. In addition, morphological alterations were determined by Transmission electron microscope.