A validated method development for ketoprofen by a flow-injection analysis with UV-detection and its application to pharmaceutical formulations.

Aboul-Enein H. Y., Dal A. G., TUNÇEL M.

Farmaco (Societa chimica italiana : 1989), vol.58, no.6, pp.419-22, 2003 (Peer-Reviewed Journal) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 58 Issue: 6
  • Publication Date: 2003
  • Doi Number: 10.1016/s0014-827x(03)00061-2
  • Journal Name: Farmaco (Societa chimica italiana : 1989)
  • Page Numbers: pp.419-22
  • Keywords: Determination of ketoprofen, Flow-injection analysis, Non-steroidal anti-inflammatory agent, Pharmaceutical analysis
  • Anadolu University Affiliated: Yes


A simple, sensitive and fast flow-injection analysis method with UV-detection method was developed for the determination of ketoprofen in pharmaceuticals. The standard and sample solutions were dissolved in a 10% ethanol which was suitable for this study. A flow-rate of 0.6 ml min-1 was used and the analyte was monitored at 260 nm. Variables such as concentrations, flow rate of reagents and other flow injection parameters were optimized to produce the most sensitive and reproducible results. Linear calibration curves were obtained in the range of 1.6×10-6 and 1.7×10-4 M. Limit of detection and limit of quantification were 1.7×10-6 M (S/N=3.3) and 5.3×10-6 M (S/N=10), respectively. The method was applied successfully to the analysis of ketoprofen pharmaceutical tablets. The recoveries were 102.75% for peak area and 98.42% for peak height. The proposed method is fast, precise, sensitive and easy to use for the determination of ketoprofen in pharmaceuticals. © 2003 Éditions scientifiques et médicales Elsevier SAS. All rights reserved.