Stability indicating determination of Hydrochlorothiazide and Triamterene by capillary electrophoresis
ACTA CHROMATOGRAPHICA, cilt.38, sa.1, ss.65-72, 2026 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 38 Sayı: 1
- Basım Tarihi: 2026
- Doi Numarası: 10.1556/1326.2025.01305
- Dergi Adı: ACTA CHROMATOGRAPHICA
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Biotechnology Research Abstracts, CAB Abstracts, Veterinary Science Database
- Sayfa Sayıları: ss.65-72
- Anahtar Kelimeler: capillary electrophoresis, Hydrochlorothiazide, micellar electrokinetic capillary chromatography, stability indicating method, Triamterene
- Anadolu Üniversitesi Adresli: Evet
Özet
In this study, a simple, fast, and sensitive stability indicating CE method was developed for the quantification of Triamterene (TRM) and Hydrochlorothiazide (HCT) in pharmaceutical preparations. Detection was achieved using a photo diode array detector, and the separation process was conducted in a capillary with an inner diameter of 75 mu m and a length of 40 cm. Optimum conditions were achieved by using a run buffer of 15 mM borate containing 20 mM SDS at pH 9.00, and applying a potential of 25 kV. HCT and TRM exhibited migration times of approximately 2.75 +/- 0.007 min and 4.61 +/- 0.022 min, respectively, under these conditions. The developed method was validated by assessing parameters such as linearity, selectivity, precision, accuracy, sensitivity, stability, and robustness. To assess the selectivity of the method, HCT, TRM, and tablets containing these compounds were exposed to acidic, basic, oxidative, thermal, and photolytic stress conditions. Ultimately, the method was proved successfully in accurately determining HCT and TRM levels in tablets.