This mixed method study aims to examine the relationship between big-five personality traits and workplace spirituality from a managerial perspective by analyzing its potential effects on management. In the quantitative step, the cross-sectional survey was employed as the data collection, and the data were obtained from a sampling group through the simple random sampling. Further, the qualitative part of the study was designed as a purposive sampling technique. The quantitative research data were obtained from 238 participants working in a public university in Turkey. The qualitative data were obtained by interviewing a group of 14 people from the same sample of participants working as administrators at the same university. The qualitative data of the study were analyzed by content analysis. The findings indicate that the harmony between the perception of personality structures and workplace spirituality has an important function in the adoption of workplace values by the employees.