One-dimensional titanium dioxide (TiO2) nanotubes (TNTs) doped with silver (Ag) (% 5) were synthesized via two-step hydrothermal method. These nanotubes were characterized by X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), Raman spectroscopy, transmission electron microscopy (TEM) and scanning electron microscopy (SEM). The impact of Ag-doped TiO2 nanotubes as antibacterial agents against three strains of Gram positive Staphylococcus aureus and a strain of Gram negative Escherichia coli was also investigated. XRD, FT-IR and Raman spectroscopy results showed that nanotubes were complete anatase phase. The potential biocidal effect of nanotubes to different S. aureus strains and E. coli was obtained. (C) 2018 Elsevier B.V. All rights reserved.