Flowering aerial parts of Stachys citrina Boiss. et Heldr. subsp. citrina (Lamiaceae) being endemic for Turkey were subjected to hydrodistillation to obtain the essential oil which was further analysed by GC-FID and GC-MS, simultaneously. 36 constituents were determined in the oil, where a-pinene (29.8%) and β-phellandrene (13.6%) were identified as the main components. The anticandidal and antibacterial effects of the oil were evaluated by using microdilution method. The oil showed weak inhibitory effects against the tested bacteria panel (1.25 to 5.0 mg/mL, MIC) whereas the Candida strains were inhibited at lower concentrations of the oil. Especially C. glabrata was inhibited at a concentration of 0. 078 mg/mL of the oil.