Chemical composition and mosquitocidal activity of n-hexane and methanolic extracts from Euphorbia anacampseros var. tmolea: An endemic species of Turkey against Aedes aegypti

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Demiray H., Tabanca N., GÖGER F., Estep A. S., Becnel J. J., DEMİRCİ B.

Asian Journal of Chemistry, vol.29, no.11, pp.2488-2492, 2017 (Scopus) identifier

  • Publication Type: Article / Article
  • Volume: 29 Issue: 11
  • Publication Date: 2017
  • Doi Number: 10.14233/ajchem.2017.20813
  • Journal Name: Asian Journal of Chemistry
  • Journal Indexes: Scopus
  • Page Numbers: pp.2488-2492
  • Keywords: Aliphatic esters, Biopesticide, Euphorbiaceae, Monoterpenes, Mosquito control
  • Anadolu University Affiliated: Yes


Volatile composition of the n-hexane and methanol extracts from E. anacampseros var. tmolea was analyzed by head space-solid phase microextraction (HS-SPME) coupled with gas chromatography-mass spectrometry (GC-MS). Chemical characterization of the methanolic extract was determined by LC-ESI-MS/MS. Both extracts were bioassayed against 1st instar larvae and adult female Ae. aegypti. The main components identified from the E. anacampseros var. tmolea n-hexane fraction were 1,8-cineole (27.5 %), p-cymene (25 %), ?-terpinene (12.8 %), limonene (9.9 %). Methyl hexanoate (18.8 %), methyl nonanoate (13.3 %), dimethyl succinate (6.5 %), methyl octanoate (6 %) and methyl phenylacetate (5.3 %) were identified from the methanolic fraction. The n-hexane extract showed 100 % mortality at 0.1 μg/ μL against 1st instar larvae of Ae. aegypti and the methanolic extract exhibited 83.3 % mortality at 5 μg/mosquito against adult female Ae. aegypti. The bioassay-guided study demonstrates that n-hexane and methanol extracts of E. anacampseros var. tmolea contain compounds with natural mosquito larvicidal and adulticidal activity.