© 2022 University of Ankara. All rights reserved.Objective: Determination and comparison of the biological activities of black and white chia seeds in extracts obtained by different extraction techniques. Material and Method: Biological activity studies of extracts obtained by different techniques were determined by applying DPPH radical scavenging potential test, TEAC test and ORAC test. In addition, total phenol and flavonoid amounts were determined in methanolic extract samples. Result and Discussion: In this study, phenol amount was determined as 9.86 mg GAE/g for black seed and 12.69 mg GAE/g for white seed and the total amount of flavonoids was determined as 0.098 mg RE/mL for black chia seed and 0.099 RE/mL for white seed. In addition, considering the antioxidant activity tests, the highest antioxidant activity for TEAC test was found in both black and white seed extracts obtained by the Cold press method, the highest activity for DPPH was in the white seed extracted obtained by the Cold press method, and the highest activity for ORAC test was observed in the white seed extract obtained by the Folch technique.