In vitro alpha-Glucosidase and alpha-Amylase Enzyme Inhibitory Effects in Elaeagnus angustifolia Leaves Extracts

SALTAN F. Z., OKUTUCU B., CANBAY H. S., Ozel D.

EURASIAN JOURNAL OF ANALYTICAL CHEMISTRY, vol.12, no.2, pp.117-126, 2017 (ESCI) identifier identifier

  • Publication Type: Article / Article
  • Volume: 12 Issue: 2
  • Publication Date: 2017
  • Doi Number: 10.12973/ejac.2017.00158a
  • Journal Name: EURASIAN JOURNAL OF ANALYTICAL CHEMISTRY
  • Journal Indexes: Emerging Sources Citation Index (ESCI), Scopus
  • Page Numbers: pp.117-126
  • Keywords: Elaeagnus angustifolia, RP-HPLC, phenolic, ASE, PHENOLIC-COMPOUNDS, L. LEAF, ANTIOXIDANT, IDENTIFICATION, COMPONENTS, SEEDS, ACID
  • Anadolu University Affiliated: Yes

Abstract

This study was performed to demonstrate alpha-glucosidase and alpha-amylase enzyme inhibitor activities using the methanolic extracts of Elaeagnus angustifolia leaves. Methanolic extracts were prepared by two different extraction methods [accelerated solvent extractor (ASE) and Soxhlet apparatus]. The analytical procedure involved the application of liquid chromatography. Our results showed that the ASE extracts inhibited a-amylase (10-100 mu g/ml) and alpha-glucosidase (10-50 mu g/ml) dose-dependently exhibitedrp inhibitory activities with the extracts [alpha-amylase (40%) and alpha-glucosidase (56%)]. RP-HPLC analyses of the methanolic extracts were detected with vanillic acid and 4-hydroxybenzoic acid as the main phenolic acids in all the ASE extracts. Elaeagnus angustifolia leaf extracts may be attributed to the presence of vanillic acid and 4-hydroxybenzoic acid, there by confirming with its traditional use for the management of diabetes mellitus. We implicate that the anti-diabetic activity through in vitro assessments and support to the folkloric use of this plant for controlling type-2 diabetes mellitus in Turkey.